Zephyr® G3 SPE Liquid Handler Workstation

The Zephyr® G3 SPE workstation is a simple and efficient liquid handler that automates the critical steps required in high-throughput solid phase extraction (SPE). It is designed to meet the needs of clinical research, forensic, pharmaceutical, and biotech laboratories. Designed to process 96 samples simultaneously with its 96 channel high volume head, the Zephyr G3 SPE workstation can process one plate in approximately 30 minutes.

Advanced analytical tools such as mass spectrometry are meeting the increasing demand for greater speed, sensitivity and specificity in toxicological screening. However, conventional sample preparation methods like SPE have not kept pace and have become a throughput limiting bottleneck in many laboratories. The Zephyr G3 SPE liquid handler eliminates bottlenecks, so that your lab can realize the full benefits of today’s powerful and costly-analytical instruments. It automatically checks for clogs at each process step. Zephyr G3 SPE workstation methods offer total portability and can be easily transferred from one workstation to another or from one site to another.

Verified Zephyr G3 SPE workstation methods can be easily transferred from one workstation to another or from one site to another, offering total portability. All of the critical steps needed to perform SPE have been automated including:

  • Plate conditioning
  • Addition of buffer and/or instrument standards
  • Loading the sample
  • Plate rinsing
  • Elution
Height660 mm | 26 in
Width762 mm | 30 in
Depth585 mm | 23 in
Contact us to automate your high-throughput SPE
For research use only. Not for use in diagnostic procedures.


Liquid Handling OptionsVolume RangeCharacteristic PerformanceTip Options
HV Pipetting Head
(96 Channels)
1-200 μL1-5 μL: CV < 5%
5-200 μL: CV < 2%
100 or 200 μL disposable tips
LV Pipetting Head
(384 Channels)
0.5-25 μL0.5-2 μL: CV < 8%
2-25 μL: CV < 5%
96,384 deep, or 384/1536 fixed cannula arrays 96 or 384 mandrel
arrays for disposable tips Custom V&P Scientific Pin tools
Height: 660 mm | 26 in
Width: 762 mm | 30 in
Depth: 585 mm | 23 in

Operating Temperature 15 – 35°C (59 to 95°F)
Operating Humidity 0 – 85% RH, non-condensing
Air Supply Regulated 65 psi

Power Input 115 VAC, 50/60 Hz, 1000 VA max.
230 VAC, 50/60 Hz, 1000 VA max.

Publications that Cite Using the Zephyr® G3 SPE Workstation

Hutchison, R. D., Ford, B. M., Franks, L. N., Wilson, C. D., Yarbrough, A. L., Fujiwara, R., . . . Prather, P. L. (2018). Atypical Pharmacodynamic Properties and Metabolic Profile of the Abused Synthetic Cannabinoid AB-PINACA: Potential Contribution to Pronounced Adverse Effects Relative to Δ9-THC. Frontiers in Pharmacology,9. doi:10.3389/fphar.2018.01084

Palcic, J. D., Donovan, S. F., Jones, J. S., Flagg, E. L., Salonga, R. A., Mock, W. E., & Asirvatham, V. S. (2016). Lewisite Metabolites in Urine by Solid Phase Extraction—Dual Column Reversed-Phase Liquid Chromatography—Isotope Dilution Tandem Mass Spectrometry. Journal of Analytical Toxicology, 40(6), 419-426. doi:10.1093/jat/bkw049

Patton, A. L., Jones, J. O., Nord, A., Eversole, D., Feazell, E. E., Mauldin, K., . . . Moran, J. H. (2018). Multi-laboratory validation of a Δ9-tetrahydrocannabinol LC-MS/MS test kit designed for quantifying THC and marijuana metabolites in blood. Forensic Science and Criminology, 3(1). doi:10.15761/fsc.1000125.