Zephyr® G3 NGS Workstation

The Zephyr® G3 NGS workstation is a benchtop liquid handler designed to automate the construction of 48 to 96 next generation sequencing (NGS) libraries per day. The simplified user-interface and integrated hardware maximize laboratory productivity while reducing variability resulting from manual pipetting.

Over 40 automated methods for the Zephyr® G3 NGS liquid handler are available automating:

  • Post-PCR SPRI purification
  • qPCR setup (in 96-well or 384-well plates)
  • Plate transfer to the LabChip® GX Touch nucleic acid analyzer
  • Volume and/or concentration normalization
  • Indexed library pooling
  • NGS library construction

The Zephyr® G3 NGS workstation produces sequence-ready libraries with improved reproducibility and consistency while minimizing the labor-intensive steps associated with manual NGS sample preparation. With a walk-away solution and flexible sample processing (8 to 96 samples), the Zephyr® G3 NGS workstation is an ideal solution for medium throughput labs enabling the parallel processing of 96 samples in less than eight hours.

The Zephyr® G3 NGS workstation comes pre-configured with:

  • 12 position deck
  • Simultaneous pipetting and gripper movement for improved efficiency
  • 4-110°C thermal heater
  • Combined 4-70°C thermal heater and shaker
  • Disposable tip waste chute
  • Fully enclosure workstation
  • Guided application user interface
  • Verified, pre-developed application templates
  • Optional UV light
Height660 mm | 26 in
Width762 mm | 30 in
Depth585 mm | 23 in
Contact us to automate your NGS library prep
For research use only. Not for use in diagnostic procedures.
Switch and Save Library Prep
Liquid Handling OptionsVolume RangeCharacteristic PerformanceTip Options
HV Pipetting Head
(96 Channels)
1-200 μL1-5 μL: CV < 5%
5-200 μL: CV < 2%
100 or 200 μL disposable tips
Height: 660 mm | 26 in
Width: 762 mm | 30 in
Depth: 585 mm | 23 in

Operating Temperature 15 – 35°C (59 to 95°F)
Operating Humidity 0 – 85% RH, non-condensing
Air Supply Regulated 65 psi

Power Input 115 VAC, 50/60 Hz, 1000 VA max.
230 VAC, 50/60 Hz, 1000 VA max.

Publications that Cite Using the Zephyr® G3 NGS Workstation

Bellott, D. W., Cho, T., Hughes, J. F., Skaletsky, H., & Page, D. C. (2018). Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures. Nature Protocols, 13(4), 787-809. doi:10.1038/nprot.2018.019.

Brandstetter, B., Dalwigk, K., Platzer, A., Niederreiter, B., Kartnig, F., Fischer, A., . . . Karonitsch, T. (2019). FOXO3 is involved in the tumor necrosis factor-driven inflammatory response in fibroblast-like synoviocytes. Laboratory Investigation. doi:10.1038/s41374-018-0184-7

Griss, J., Bauer, W., Wagner, C., Maurer-Granofszky, M., Simon, M., Chen, M., . . . Wagner, S. N. (2018). B cells sustain inflammation and improve survival in human melanoma:. doi:10.1101/478735.

Gösch, L., et al. (2018) A T cell-specific deletion of HDAC1 protects against experimental autoimmune encephalomyelitis. Journal of Autoimmunity. (86).

Klumpp, J., Staubli, T., Schmitter, S., Hupfeld, M., Fouts, D. E., & Loessner, M. J. (2014). Genome Sequences of Three Frequently Used Listeria monocytogenes and Listeria ivanovii Strains. Genome Announcements, 2(2). doi:10.1128/genomea.00404-14.

Li, Jin, et al. (2017) Artemisinins Target GABAA Receptor Signaling and Impair a Cell Identity. Cell. 168 (1) 86 – 100.e15.

Skucha, A., Ebner, J., Schmöllerl, J., Roth, M., Eder, T., César-Razquin, A., . . . Grebien, F. (2018). MLL-fusion-driven leukemia requires SETD2 to safeguard genomic integrity. Nature Communications,9(1). doi:10.1038/s41467-018-04329-y.

Trolle, C., et al. (2016) Widespread DNA hypomethylation and differential gene expression in Turner syndrome. Scientific Reports (6) doi:10.1038/srep34220 .