AUTOMATED LIQUID HANDLING FOR NGS LIBRARY CONSTRUCTION

Zephyr® G3 NGS Workstation

The Zephyr® G3 NGS workstation is a benchtop liquid handler designed to automate the construction of 48 to 96 next generation sequencing (NGS) libraries per day. The simplified user-interface and integrated hardware maximize laboratory productivity while reducing variability resulting from manual pipetting.

Over 40 automated methods for the Zephyr® G3 NGS liquid handler are available automating:

  • Post-PCR SPRI purification
  • qPCR setup (in 96-well or 384-well plates)
  • Plate transfer to the LabChip® GX Touch™ nucleic acid analyzer
  • Volume and/or concentration normalization
  • Indexed library pooling
  • NGS library construction

The Zephyr® G3 NGS workstation produces sequence-ready libraries with improved reproducibility and consistency while minimizing the labor-intensive steps associated with manual NGS sample preparation. With a walk-away solution and flexible sample processing (8 to 96 samples), the Zephyr® G3 NGS workstation is an ideal solution for medium throughput labs enabling the parallel processing of 96 samples in less than eight hours.

The Zephyr® G3 NGS workstation comes pre-configured with:

  • 12 position deck
  • Simultaneous pipetting and gripper movement for improved efficiency
  • 4-110°C thermal heater
  • Combined 4-70°C thermal heater and shaker
  • Disposable tip waste chute
  • Fully enclosure workstation
  • Guided application user interface
  • Verified, pre-developed application templates
  • Optional UV light
Zephyr G3 NGS workstation
Zephyr G3 NGS liquid handler
Dimensions
Height660 mm | 26 in
Width762 mm | 30 in
Depth585 mm | 23 in
Contact us to automate your NGS library prep
For research use only. Not for use in diagnostic procedures.
Pipetting:
Liquid Handling OptionsVolume RangeCharacteristic PerformanceTip Options
HV Pipetting Head
(96 Channels)		1-200 μL1-5 μL: CV < 5%
5-200 μL: CV < 2%		100 or 200 μL disposable tips
Dimensions:
Height: 660 mm | 26 in
Width: 762 mm | 30 in
Depth: 585 mm | 23 in

Operating Temperature 15 – 35°C (59 to 95°F)
Operating Humidity 0 – 85% RH, non-condensing
Air Supply Regulated 65 psi

Power Input 115 VAC, 50/60 Hz, 1000 VA max.
or
230 VAC, 50/60 Hz, 1000 VA max.

Publications that Cite Using the Zephyr® G3 NGS Workstation

Bellott, D. W., Cho, T., Hughes, J. F., Skaletsky, H., & Page, D. C. (2018). Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures. Nature Protocols, 13(4), 787-809. doi:10.1038/nprot.2018.019.

Brandstetter, B., Dalwigk, K., Platzer, A., Niederreiter, B., Kartnig, F., Fischer, A., . . . Karonitsch, T. (2019). FOXO3 is involved in the tumor necrosis factor-driven inflammatory response in fibroblast-like synoviocytes. Laboratory Investigation. doi:10.1038/s41374-018-0184-7

Griss, J., Bauer, W., Wagner, C., Maurer-Granofszky, M., Simon, M., Chen, M., . . . Wagner, S. N. (2018). B cells sustain inflammation and improve survival in human melanoma:. doi:10.1101/478735.

Gösch, L., et al. (2018) A T cell-specific deletion of HDAC1 protects against experimental autoimmune encephalomyelitis. Journal of Autoimmunity. (86). doi.org/10.1016/j.jaut.2017.09.008.

Klumpp, J., Staubli, T., Schmitter, S., Hupfeld, M., Fouts, D. E., & Loessner, M. J. (2014). Genome Sequences of Three Frequently Used Listeria monocytogenes and Listeria ivanovii Strains. Genome Announcements, 2(2). doi:10.1128/genomea.00404-14.

Li, Jin, et al. (2017) Artemisinins Target GABAA Receptor Signaling and Impair a Cell Identity. Cell. 168 (1) 86 – 100.e15.

Skucha, A., Ebner, J., Schmöllerl, J., Roth, M., Eder, T., César-Razquin, A., . . . Grebien, F. (2018). MLL-fusion-driven leukemia requires SETD2 to safeguard genomic integrity. Nature Communications,9(1). doi:10.1038/s41467-018-04329-y.

Trolle, C., et al. (2016) Widespread DNA hypomethylation and differential gene expression in Turner syndrome. Scientific Reports (6) doi:10.1038/srep34220 .