AUTOMATED LIQUID HANDLING PLATFORM FOR SMALL-SCALE PROTEIN PURIFICATION APPLICATIONS
JANUS® G3 BioTX™ Pro Workstations
The JANUS® G3 BioTx™ automated workstations enable consistent small-scale protein purification and sample prep for analytical protein characterization required to support quality by design experimentation in both upstream and downstream processes. Automating sample prep provides researchers more time to focus on new analytical tests obtaining critical information earlier in the protein development pipeline. Time and labor savings accelerate project workflows and thus, commercialization of protein therapies.
Automate Four Modes of Small-scale Protein Purification on a Single Platform
- PhyNexus® PhyTips® columns
- GE® PreDictor™ plates/ Pall® AcroPrep™ filter plates
- OPUS® RoboColumns® Chromatography Columns
- GE® His GraviTrap™ columns
Maximize Productivity with Automated Purification Methods
The JANUS® G3 BioTx™ workstations accommodate column, tip, and batch chromatography modes on one platform. With just one instrument, you can achieve rapid analysis of a range of sample volumes and concentrations to support Quality by Design experimentation in both upstream or downstream processes.
To further maximize productivity PerkinElmer has created the PKeye™ Mobile Operations Monitor, which allows for true walk-away push button protocols through advanced monitoring with integrated cameras. Learn more here.

PhyNexus® PhyTips® Columns
- Purify up to 96 samples in 15 min
- Column bed sizes from 5-160 μL volumes
- Achieve high sample binding capacity and purity by passing samples back and forth through column
- Highly purified products with high concentrations achieved with low elution:bed volume ratio

GE® Predictor® Plates/Pall® AcroPrep™ Filter Plates
- Rapidly screen binding, wash and elution conditions in 96 well plate formats
- Use centrifugation or vacuum filtration (optional gripper for full automation)
- Explore design space to optimize process development using DoE

OPUS® RoboColumns® Chromatography Columns
- Miniaturized column chromatography predictive of process scale-up
- Purify 96 samples/day or more depending upon load volume
- 50, 100, 200 and 600 μL bed sizes; variety of resin types
- Sample volumes from 100 μL up to 48 mL or more
- Automate sample loading and fraction collection; adjustable height accommodates multiple collection plates
Figure 1. The JANUS® G3 BioTx™ workstation accommodates (A) PhyNexus® PhyTips® columns, (B) filter plates and (C) OPUS® RoboColumns® Chromatography Columns on one platform. Purification methods include affinity, ion exchange, reverse phase and gel filtration.
Simple to Use Protocols—Designed for the Scientist

Quickly enter experiment variables to easily translate your experimental design.

Easily confirm placement of samples and labware on the workstation deck (top) and view workstation status or setup email confirmation for when process is complete (bottom).

Wizard driven JANUS® G3 application assistant guides preparation and execution of small-scale purification and sample prep
- Enables true walk-up usability without need for advanced training
- Flexibility to easily modify purification parameters

Choose the JANUS® G3 BioTx™ Pro workstation or the JANUS® G3 BioTx™ Pro Plus Workstation Depending upon Your Throughput and Capacity Needs
- Eight tip independent pipetting arm designed for multiple
protein chromatography applications:
PhyNexus® PhyTips® columns, GE® Predictor® Plates/Pall®
AcroPrep™ filter plates, OPUS® RoboColumns®
chromatography columns, GE® His GraviTrap™ columns - Flexible control software and wizard driven application assistant
- Automated fraction collection station with housing for up to 96 small scale columns

JANUS® G3 BIOTX™ PROWORKSTATION
- 12 deck positions
- Compact footprint

JANUS® G3 BIOTX™ PRO PLUS WORKSTATION
- 24 deck locations
- MDT 96-tip head for parallel purification methods
- Integrated gripper for labware positioning
- Integrated vacuum manifold
Precision Automated Pipetting with a Wide Dynamic Volume Range:
Pipetting Arm | Volume | % CV | Tip/Head | Condition |
---|---|---|---|---|
Varispan | 5 µL | < 2% | 20 µL disposable tip | Distilled water, 500 µL syringe |
50 µL | < 1% | Fixed tip | Distilled water, 500 µL syringe | |
MDT | 1 µL | < 2% | I20 disposable tip on I50 96-tip Head | 1 µL |
1 µL | < 5% | I50 disposable tips on I50 96-tip Head | 1 µL | |
5 µL | < 1% | I50 disposable tips on I50 96-tip Head | 5 µL | |
50 µL | < 1% | I235 disposable tips on I235 96-tip Head | 50 µL |
JANUS G3 Platform | JANUS® G3 BioTx Pro Workstation Configuration | JANUS® G3 BioTx Pro Plus Workstation Configuration |
---|---|---|
Varispan | 9 plates | N/A |
Varispan + MDT | N/A | 20 plates |
Dimensions | ||
---|---|---|
JANUS® G3 Workstations Without an Enclosure | ||
Platform | JANUS G3 BioTx Pro Workstation | JANUS G3 BioTx Pro Plus Workstation |
Varispan 8-tip | H: 864 mm | 34 in W: 788 mm | 31 in D: 838 mm | 33 in | N/A |
Varispan + MDT | N/A | H: 1118 mm | 44 in W: 1499 mm | 59 in D: 838 mm | 33 in (Includes space requirements for plate::shuttle) |
Publications that Cite Using the JANUS® G3 BioTX™ Pro Workstations for Protein Purification
Gengenbach, B., Müschen, C., & Buyel, J. (2018). Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems. Protein Expression and Purification, 151, 46-55. doi:10.1016/j.pep.2018.06.003
van der Veen, A. G., Maillard, P. V., …, Snijders, A. P. (2018). The RIG‐I‐like receptor LGP2 inhibits Dicer‐dependent processing of long double‐stranded RNA and blocks RNA interference in mammalian cells. EMBO, 37(12). Doi:10.15252/embj.201797479.
SCIENTIFIC POSTERS
FLYERS
- JANUS® G3 Workstation Spec Sheet
- Your Complete Solution for Sample Prep in Process Development & Protein Characterization
- Large Volume Dispensing for JANUS® Workstations
PRODUCT NOTE
APPLICATION NOTE