Assay development in the drug discovery process is a time intensive process. This process requires a great deal of iteration and manual labor. Determining the correct assay conditions and specifications carries a certain amount of risk, since it requires significant knowledge and time investment. To combat these issues, researchers use many different technologies and platforms. With the JANUS G3 Assay Development workstation, we developed an end-to-end platform that is fully integrated, automated, and optimally configured, for protein-protein interaction (PPI) assays utilizing homogeneous time-resolved fluorescence (HTRF®).
This fully integrated solution requires zero programing knowledge or automation experience. This platform has been optimized to remove risk associated with assay development and systemically optimized for PPI assays.
This assay development solution utilizes TIBCO® Spotfire software (included in PerkinElmer Signals VitroVivo software), the JANUS G3 Assay Development workstation, the VICTOR Nivo multimode plate reader and the Signals VitroVivo software. The Spotfire software provides a plate map and step-by-step transcript for the JANUS G3 Assay Development Workstation. The workstation automates the pipetting and plate reading on the VICTOR Nivo plate reader. The PerkinElmer Signals VitroVivo recommendations engine performs data analysis on the assay and makes suggestions for the optimal combination and concentrations of reagents for further development.