FULLY INTEGRATED PLATFORM FOR ASSAY DEVELOPMENT IN DRUG DISCOVERY
JANUS® G3 Assay Development Workstation
Assay development in the drug discovery process is a time intensive process. This process requires a great deal of iteration and manual labor. Determining the correct assay conditions and specifications carries a certain amount of risk, since it requires significant knowledge and time investment. To combat these issues, researchers use many different technologies and platforms. With the JANUS G3 Assay Development workstation, we developed an end-to-end platform that is fully integrated, automated, and optimally configured, for protein-protein interaction (PPI) assays utilizing homogeneous time-resolved fluorescence (HTRF®).
This fully integrated solution requires zero programing knowledge or automation experience. This platform has been optimized to remove risk associated with assay development and systemically optimized for PPI assays.
This assay development solution utilizes TIBCO® Spotfire software (included in our Signals VitroVivo software), the JANUS G3 Assay Development workstation, the VICTOR Nivo multimode plate reader and the Signals VitroVivo software. The Spotfire software provides a plate map and step-by-step transcript for the JANUS G3 Assay Development Workstation. The workstation automates the pipetting and plate reading on the VICTOR Nivo plate reader. Signals VitroVivo recommendations engine performs data analysis on the assay and makes suggestions for the optimal combination and concentrations of reagents for further development.
JANUS G3 Assay Development Workstation
The JANUS G3 Assay Development workstation deck is optimized for the assay development process. This workstation can autonomously prepare even the most complex assay plate in either a 96 or 384 well plate. The JANUS G3 Assay Development workstation is equipped with:
- VariSpanTM pipetting arm, to prepare assay plates accurately and precisely with channel independent liquid level detection
- Integrated gripper, to move assay plates and labware across the deck and in and off the VICTOR Nivo plate reader
- VICTOR Nivo plate reader, to read the assay plate
- Plate shaking capability, to ensure accurate mixing in well plates
- Expanded deck, to enable assay plate preparation of the most complex workflows
VICTOR Nivo Plate Reader
The seamlessly integrated VICTOR Nivo plate reader will take the assay plate and software plate map to generate GMP compliant data from the assay. The plate reader features temperature and gas control and is based on laser alpha technology. The VICTOR Nivo plate reader can measure any wavelength with standardized top and bottom well reading of factors such as:
- Absorbance
- Luminescence
- Fluorescence intensity
- Time-resolved fluorescence
- Fluorescence polarization
- Alpha