AUTOMATED METHODS

For the Sciclone®, JANUS® and Zephyr® G3 Liquid Handlers

Automated NGS Applications

Large Breadth of NGS Applications Available

More than 150 standardized and tested automated methods are available for the most frequently used NGS library prep kits. This extensive library of standardized and tested automated methods offers flexibility in the choice of kits. Together, with our suite of automated liquid handling solutions optimized for NGS applications, the PerkinElmer NGS application library provides the ability to adapt to your needs.


Emerging Applications

PerkinElmer’s team of application scientists is continuously releasing new automated methods to address emerging applications. By being responsive to latest trends in the NGS field, you can be assured we can meet your future automation needs.


Advanced Error Handling

PerkinElmer enables true walk-away push button protocols with integrated on-deck cameras through the PKeye™ mobile operations monitor. This system will notify you of any errors that occur through the PKeye™ cloud-based platform, allowing you to walk away from the workstation and focus your attention on intellectual contributions to the lab.

///// The table below lists the kits and protocols that are currently automated on the Sciclone®, JANUS®, and Zephyr® G3 liquid handlers.

INCREASE YOUR THROUGHPUT & REPRODUCIBILITY WITH AUTOMATED NGS METHODS

DNA Library Prep Kits
  SCICLONE G3 NGSxSCICLONE NGSx iQSCICLONE NGSx HTZEPHYR G3 NGS
PerkinElmerNEXTFLEX® Rapid XP DNA-Seq Pre-plated Automation KitXXXX
PerkinElmerNEXTFLEX® Rapid DNA-Seq Kit 2.0XX
PerkinElmerNEXTFLEX® Rapid DNA-Seq KitX
PerkinElmerNEXTFLEX® Cell Free DNA-Seq Kit 2.0XX
PerkinElmerPG-Seq® KitX
PerkinElmerNEXTFLEX® Rapid 2.0X
PerkinElmerNEXTFLEX® Cell Free DNA-Seq Kit 2.0X
10X Genomics®Genomic PreparationX
ArcherDx®Universal DNA Reagent Kit v2X
CareDx®AlloSeq TZX
IDT®Lotus DNA Library Prep
X
Illumina®Nextera® XT DNA KitXXX
Illumina®TruSeq® DNA PCR-Free KitX
Illumina®TruSeq® Nano DNA KitXX
Illumina®DNA Prep KitXX
Illumina®DNA Prep with EnrichmentXXX
DNA PCR-Free Library PrepTagmentationX
NEB®NEBNext® Ultra II DNA Library Prep for Illumina® InstrumentsXXX
NEB®NEBNext® Ultra DNA KitXX
NEB®NEBNext® dsDNA Fragmentase KitX
NuGen®Ovation® Ultralow DR Multiplex System (1-96)XX
PacBio®2kb Template Preparation & SequencingX
PacBio®10kb Template Preparation & SequencingXXX
PacBio®20kb Template Preparation Using BluePippin Size-Selection SystemXX
PacBio®Greater than 10kb Template Preparation Using AMPure® PB BeadsXX
Thermo Fisher®Ion Torrent Ion Express® Fragment Library Prep KitXX
Thermo Fisher®Ion Express® Plus gDNA Fragment Library Prep Kit
TwinStrandBioscience
TwinStrand Duplex Sequencing: Pre-PCRX
TwinStrandBioscience
TwinStrand Duplex Sequencing: Post-PCRX
Twist®Library Preparation KitXX
QIAGEN®QIAseq® FX DNA Library KitX
QIAGEN®QIAGEN®Targeted DNA v3 PanelXX
Swift BiosciencesAccel-NGS® 2S Plus DNA Library KitX
Swift BiosciencesNormalaseAmplicon Panels (SNAP)X
Roche®DNA HyperPrep™ KitXX
Roche®DNA HyperPlus™ KitXX
Roche®KAPA HyperCap v3XX
Roche®DNA HTP Library Preparation KitX
Exome / target capture library prep kits
  SCICLONE G3 NGSSCICLONE NGSx iQZEPHYR G3 NGS
PerkinElmerNEXTFLEX® Variant Seq™ SARS-CoV-2 Kit
XX
PerkinElmerNEXTFLEX®Variant Seq™ SARS-CoV-2 V2 KitXXX
10x Genomics®Exome PreparationX
Agilent®HaloPlex® KitX
Agilent®SureSelect® XT2 KitXXX
Agilent®SureSelect® XT KitXX
Agilent®SureSelect® QXT KitXX
Agilent®SureSelect® XT HSXX
Cergentis® TLA Targeted Locus Amplification KitX
IDT®/EpicentreXGen® Exome Capture KitXX
Illumina®COVIDSeq™ TestXX
Illumina®Nextera® Rapid Capture KitXX
Illumina®TruSeq® Rapid Exome KitX
Illumina®TruSeq® Custom Amplicon v1.5 KitX
Illumina®TruSeq® Custom Amplicon Low Input KitXX
Illumina®TruSeq® Exome KitX
Illumina®TruSeq® Focus PanelX
Illumina®TruSeq® Cancer HotSpot Panel v2X
Illumina®TruSeq® Comprehensive Panel v3X
Illumina®TruSight® Cancer KitX
Illumina®TruSight® Enrichment Kit
Illumina®TruSight® Rapid Capture KitX
Illumina®TruSight® Tumor Kit
Illumina®TruSight® Tumor 15 Kit
NEB®NEBNext Direct® Cancer HotSpot PanelX
NuGen®Ovation® Target Enrichment SystemX
Roche®SeqCap® EZ KitX
Roche®SeqCap® EZ HyperPrep® KitX
Thermo Fisher®Ion TargetSeq Exome Kit
Thermo Fisher®Ion Ampliseq® Panels
Twist®Human Core Exome KitXX
Twist®TWIST® Custom PanelsXX
Twist®Fast Hybridization Target EnrichmentXX
Small RNA Automation Methods
  SCICLONE G3 NGSSCICLONE NGSx iQZEPHYR G3 NGS
PerkinElmerNEXTFLEX® Small RNA-Seq Kit v3XXX
PerkinElmerNEXTFLEX® Small RNA-Seq Automation Kit v3 with UDIsXX
PerkinElmerNEXTFLEX® Small RNA-Seq v3 Automation Kit with UDIsX
RNA Library Prep Kits
  SCICLONE G3 NGSSCICLONE NGSx iQSCICLONE NGSx HTZEPHYR G3 NGS
PerkinElmerNEXTFLEX® Rapid Directional RNA-Seq Kit 2.0XX
PerkinElmerNEXTFLEX® Rapid Directional RNA-Seq Kit with Poly(A) Beads 2.0XXX
PerkinElmerNEXTFLEX® Rapid Directional qRNA-Seq KitX
PerkinElmerNEXTFLEX® Rapid RNA-Seq KitXX
PerkinElmerNEXTFLEX® Small RNA-Seq Kit v3XXX
PerkinElmerNEXTFLEX® Small RNA-Seq Automation Kit v3 with UDIsXXX
PerkinElmerNEXTFLEX® Combo-Seq™ mRNA/miRNA KitXX
PerkinElmerNEXTFLEX® Rapid Directional RNA-Seq Kit 2.0X
Agilent®SureSelect® Strand-Specific RNA Library Prep Kit
Illumina®TruSeq® RNA Access KitX
Illumina®TruSeq® Stranded mRNA (poly-A selection) KitXXX
Illumina®TruSeq® Stranded Total RNA (Ribozero) KitXXX
Illumina®/EpicentreRibozero/Scriptseq Stranded RNA KitX
Roche®mRNA HyperPrep KitXX
Roche®RNA HyperPrep KitXXX
Roche®RNA HyperPrep Kit with RiboEraseXX
Roche®Stranded RNA-Seq KitXX
Roche®Stranded RNA-Seq Kit with RiboEraseXX
Roche®Stranded mRNA-Seq KitXX
Lexogen®autoSENSE mRNA-Seq Library Prep Kit for Illumina® SequencingX
Lexogen®autoQuantSeq 3'mRNA-Seq Library Prep Kit for Illumina® SequencingX
NEB®NEBNext® Ultra RNA Library Prep Kit for Illumina® SequencingXX
NuGen®NEBNext® Ultra RNA II Library Prep Kit for Illumina® SequencingX
NuGen®Ovation® Human Blood RNA-Seq Library SystemsX
NuGen®Ovation® Human FFPE RNA-Seq Multiplex SystemX
NuGen®Ovation® RNA-Seq System v2X
NuGen®Ovation® Universal RNA-Seq System (strand-specific)X
10X Genomics®Chromium Single Cell 3' Library Kit v3X
Takara formerly ClontechSMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input MammalianX
Takara formerly ClontechSMART-Seq® v4 Ultra® Low Input RNA Kit for SeqeuncingX
Takara formerly ClontechSMART-Seq® HT KitX
Thermo Fisher®(Ion Torrent) Ion Total RNA Kit v2
Twist®cDNA Library Preparation Kit for ssRNA Virus DetectionXX
QIAGEN®QIASeq®DIRECT SARS-CoV-2
XX
Metagenomics Library prep kits
  SCICLONE G3 NGSSCICLONE NGSx iQZEPHYR G3 NGS
PerkinElmerNEXTFLEX® 16S V1-V3 Amplicon-Seq KitX
PerkinElmerNEXTFLEX® 16S V4 Amplicon-Seq Kit 2.0X
PerkinElmerNEXTFLEX® 16S V5-V6 Amplicon-Seq KitX
PerkinElmerNEXTFLEX® 18S ITS Amplicon-Seq KitX
PerkinElmerNEXTFLEX® 16S V4 & 18S ITS Amplicon-Seq KitX
PerkinElmerNEXTFLEX® Rapid XP DNA-Seq KitXXX
PerkinElmerNEXTFLEX® Rapid DNA-Seq Kit 2.0XX
PerkinElmerNEXTFLEX® Rapid DNA-Seq KitX
NuGen®Ovation® Ultralow Methyl-Seq Library SystemX
Supplementary Applications and Products
  SCICLONE G3 NGSZEPHYR G3 NGS
PerkinElmerNEXTFLEX® Poly(A) Bead 2.0 SelectionXX
PerkinElmerNormalization & Pooling ProtocolXX
Beckman Coulter®SPRI® Purification ProtocolXX
Beckman Coulter®SPRI® Size Selection ProtocolXX
Roche®SYBR® FAST qPCR KitX
Roche®Library Quant KitXX
Roche®hgDNA Quantification and QC ProtocolXX
Thermo Fisher®PicoGreen® QuantitationXX
Thermo Fisher®Qubit® QuantitationX
Thermo Fisher®Quant-iT® QuantitationX
NuGen®Encore® Biotin ModuleX
NuGen®Ovation® FFPE WTA SystemX
NuGen®Ovation® Pico WTA System v2X

LIQUID HANDLERS DESIGNED TO MEET YOUR THROUGHPUT REQUIREMENTS

PerkinElmer offers a suite of liquid handlers to offer labs the flexibility of choosing a automation solution based on their throughput needs. Learn more about our liquid handlers optimized for NGS library prep.

Automate your sample preparation procedures

For hands-free automated construction
of up to or greater than 96 libraries per day

Automate your sample preparation procedures

For the automated construction of up to or
greater than 96 libraries per day

Adaptable and scalable personalized automated liquid handling solutions

For the automated construction of 48 to
96 libraries per day

Efficiently automate your sample preparation procedures

Automated construction of up to
24 libraries per day

Contact us to automate your applications

For research use only. Not for use in diagnostic procedures.