AUTOMATED METHODS
For the Sciclone® and Zephyr® G3 Liquid Handlers
Automated NGS Applications
Large Breadth of NGS Applications Available
More than 120 standardized and tested automated methods are available for the most frequently used NGS library prep kits. This extensive library of standardized and tested automated methods offers flexibility in the choice of kits. Together, with our suite of automated liquid handling solutions optimized for NGS applications, our application library provides the ability to adapt to your needs.
Emerging Applications
Our team of application scientists is continuously releasing new automated methods to address emerging applications. By being responsive to latest trends in the NGS field, you can be assured we can meet your future automation needs.
Advanced Error Handling
We enable true walk-away push button protocols with integrated on-deck cameras through the PKeye™ mobile operations monitor. This system will notify you of any errors that occur through the PKeye™ cloud-based platform, allowing you to walk away from the workstation and focus your attention on intellectual contributions to the lab.
///// The tables below lists the kits and protocols that are currently automated on the Sciclone® and Zephyr® G3 liquid handlers.
INCREASE YOUR THROUGHPUT & REPRODUCIBILITY WITH AUTOMATED NGS METHODS
| SCICLONE G3 NGSx | SCICLONE NGSx iQ | ZEPHYR G3 NGS | BIOQULE NGS SYSTEM | ||
|---|---|---|---|---|---|
| PerkinElmer | NEXTFLEX® Rapid XP V2 DNA-Seq Kit | X | X | X | X |
| PerkinElmer | NEXTFLEX® Rapid DNA-Seq Kit 2.0 | X | X | X | X |
| PerkinElmer | NEXTFLEX® Cell Free DNA-Seq Kit 2.0 | X | X | X | |
| 10X Genomics® | Genomic Preparation | X | |||
| ArcherDx® | Universal DNA Reagent Kit v2 | X | |||
| CareDx® | AlloSeq TZ | X | |||
| IDT® | xGen DNA Library Prep Kit EZ | X | |||
| Illumina® | Nextera® XT DNA Kit | X | X | X | |
| Illumina® | TruSeq® DNA PCR-Free Kit | X | X | ||
| Illumina® | TruSeq® Nano DNA Kit | X | |||
| Illumina® | DNA Prep Kit | X | X | X | |
| Illumina® | DNA Prep with Enrichment | X | X | ||
| Illumina® | DNA PCR-Free Library Prep Tagmentation | X | X | ||
| NEB® | NEBNext® Ultra™ II DNA Library Prep for Illumina® Instruments | X | X | ||
| NEB® | NEBNext® Ultra™ DNA Kit | X | X | ||
| NEB® | NEBNext® dsDNA Fragmentase™ Kit | X | |||
| NuGen® | Celero DNA-Seq | X | |||
| NuGen® | Ovation® Ultralow DR Multiplex System (1-96) | X | X | ||
| PacBio® | 2kb Template Preparation & Sequencing | X | X | X | |
| PacBio® | 10kb Template Preparation & Sequencing | X | X | X | |
| PacBio® | 20kb Template Preparation Using BluePippin™ Size-Selection System | X | X | ||
| PacBio® | Greater than 10kb Template Preparation Using AMPure® PB Beads | X | X | ||
| PacBio® | HiFi SMRTbell® Libraries with SMRTbell Prep Kit 3.0 | X | X | ||
| PacBio® | Multiplexed Microbial Library Prep with SMRTbell® Express Template Prep Kit 2.0 | X | X | ||
| Thermo Fisher® | Ion Torrent™ Ion Express® Fragment Library Prep Kit | X | X | ||
| Thermo Fisher® | Ion Express® Plus gDNA Fragment Library Prep Kit | X | X | ||
| TwinStrand™Bioscience | TwinStrand Duplex Sequencing™: Pre-PCR | X | |||
| TwinStrand™Bioscience | TwinStrand Duplex Sequencing™: Post-PCR | X | |||
| Twist® | Library Preparation Kit | X | X | ||
| QIAGEN® | QIAseq® FX DNA Library Kit | X | |||
| QIAGEN® | QIAGEN®Targeted DNA v3 Panel | X | X | ||
| IDT®/Swift® | Accel-NGS® 2S Plus DNA Library Kit | X | |||
| IDT®/Swift® | xGen Amplicon Panels | X | |||
| Roche® | DNA HyperPrep™ Kit | X | X | X | |
| Roche® | DNA HyperPlus™ Kit | X | X | X | |
| Roche® | KAPA ExoPlus Kit | X | |||
| Roche® | DNA HTP Library Preparation Kit | X | X |
| SCICLONE G3 NGS | SCICLONE NGSx iQ | ZEPHYR G3 NGS | ||
|---|---|---|---|---|
| PerkinElmer | NEXTFLEX®Variant Seq™ SARS-CoV-2 V2 Kit | X | X | X |
| Agilent® | HaloPlex® Kit | X | ||
| Agilent® | SureSelect® XT2 Kit | X | X | X |
| Agilent® | SureSelect® XT Kit | X | X | |
| Agilent® | SureSelect® QXT Kit | X | X | |
| Agilent® | SureSelect® XT HS | X | X | |
| Cergentis® | TLA Targeted Locus Amplification Kit | X | ||
| IDT®/Epicentre | xGen Hybridization and Wash Kit | X | ||
| IDT®/Epicentre | xGen Exome Hyb Panel v2 | X | X | |
| Illumina® | COVIDSeq™ Test | X | X | X |
| Illumina® | Nextera® Rapid Capture Kit | X | X | |
| Illumina® | TruSeq® Rapid Exome Kit | X | ||
| Illumina® | TruSeq® Custom Amplicon v1.5 Kit | X | ||
| Illumina® | TruSeq® Custom Amplicon Low Input Kit | X | X | |
| Illumina® | TruSeq® Exome Kit | X | ||
| Illumina® | TruSeq® Focus Panel | X | ||
| Illumina® | TruSeq® Cancer HotSpot Panel v2 | X | ||
| Illumina® | TruSeq® Comprehensive Panel v3 | X | ||
| Illumina® | TruSight® Cancer Kit | X | ||
| Illumina® | TruSight® Enrichment Kit | |||
| Illumina® | TruSight® Rapid Capture Kit | X | ||
| Illumina® | TruSight® Tumor Kit | |||
| Illumina® | TruSight® Tumor 15 Kit | |||
| NEB® | NEBNext Direct® Cancer HotSpot Panel | X | ||
| NuGen® | Ovation® Target Enrichment System | X | ||
| Roche® | KAPA HyperCap v3 | X | X | |
| Roche® | SeqCap® EZ Kit | X | ||
| Roche® | SeqCap® EZ HyperPrep® Kit | X | ||
| Thermo Fisher® | Ion TargetSeq™ Exome Kit | |||
| Thermo Fisher® | Ion Ampliseq® Panels | |||
| Twist® | Human Core Exome Kit | X | X | |
| Twist® | TWIST® Custom Panels | X | X | |
| Twist® | Fast Hybridization Target Enrichment | X | X |
| SCICLONE G3 NGS | SCICLONE NGSx iQ | ZEPHYR G3 NGS | ||
|---|---|---|---|---|
| PerkinElmer | NEXTFLEX® Small RNA-Seq Kit v3 | X | X | X |
| PerkinElmer | NEXTFLEX® Small RNA-Seq v3 Automation Kit with UDIs | X | X | X |
| SCICLONE G3 NGS | SCICLONE NGSx iQ | SCICLONE NGSx HT | ZEPHYR G3 NGS | ||
|---|---|---|---|---|---|
| PerkinElmer | NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 with Poly(A) Beads | X | X | X | |
| PerkinElmer | NEXTFLEX® Combo-Seq™ mRNA/miRNA Kit | X | X | ||
| PerkinElmer | NEXTFLEX® Small RNA-Seq Kit v3 | X | X | X | |
| PerkinElmer | NEXTFLEX® Small RNA-Seq Automation Kit v3 with UDIs | X | X | X | |
| 10X Genomics® | Chromium™ Single Cell 3' Library Kit v3 | X | |||
| Agilent® | SureSelect® Strand-Specific RNA Library Prep Kit | X | |||
| Illumina® | RNA Prep with Enrichment (L) Tagmentation | X | X | ||
| Illumina® | TruSeq® RNA Access Kit | X | |||
| Illumina® | Stranded mRNA prep | X | X | ||
| Illumina® | TruSeq® Stranded mRNA (poly-A selection) Kit | X | X | X | |
| Illumina® | TruSeq® Stranded Total RNA™ Kit | X | X | X | |
| Illumina® | Stranded Total RNA Prep, Ligation with Ribo-Zero Plus | X | |||
| Illumina® | TruSeq® RNA Library Prep Kit v2 | X | X | X | |
| Illumina®/Epicentre | Ribozero™/Scriptseq™ Stranded RNA Kit | X | |||
| Roche® | mRNA HyperPrep™ Kit | X | X | ||
| Roche® | RNA HyperPrep™ Kit | X | X | X | |
| Roche® | RNA HyperPrep™ Kit with RiboErase™ | X | X | ||
| Roche® | Stranded RNA-Seq Kit | X | X | ||
| Roche® | Stranded RNA-Seq Kit with RiboErase™ | X | X | ||
| Roche® | Stranded mRNA-Seq Kit | X | X | ||
| Lexogen® | autoSENSE™ mRNA-Seq Library Prep Kit for Illumina® Sequencing | X | |||
| NEB® | NEBNext® Ultra™ RNA Library Prep Kit for Illumina® Sequencing | X | X | ||
| NEB® | NEBNext® Ultra™ RNA II Library Prep Kit for Illumina® Sequencing | X | |||
| NuGen® | Ovation® Human Blood RNA-Seq Library Systems | X | |||
| NuGen® | Ovation® Human FFPE RNA-Seq Multiplex System | X | |||
| NuGen® | Ovation® RNA-Seq System v2 | X | |||
| NuGen® | Ovation® Universal RNA-Seq System (strand-specific) | X | |||
| Takara formerly Clontech | SMART-Seq® HT Kit | X | |||
| Thermo Fisher® | Ion Express™ Plus gDNA Fragment Library Prep Kit | X | |||
| Thermo Fisher® | (Ion Torrent™) Ion Total RNA Kit v2 | X | |||
| Twist® | cDNA Library Preparation Kit for ssRNA Virus Detection | X | X |
| SCICLONE G3 NGS | SCICLONE NGSx iQ | ZEPHYR G3 NGS | ||
|---|---|---|---|---|
| PerkinElmer | NEXTFLEX® 16S V1-V3 Amplicon-Seq Kit | X | ||
| PerkinElmer | NEXTFLEX® 16S V4 Amplicon-Seq Kit 2.0 | X | ||
| PerkinElmer | NEXTFLEX® ITS Amplicon-Seq Kit | X | ||
| PerkinElmer | NEXTFLEX® Rapid DNA-Seq Kit 2.0 | X | X | |
| PerkinElmer | NEXTFLEX® Rapid XP DNA-Seq Kit v2 | X | X | X |
| NuGen® | Ovation® Ultralow Methyl-Seq Library System | X |
| SCICLONE G3 NGS | ZEPHYR G3 NGS | ||
|---|---|---|---|
| PerkinElmer | NEXTFLEX® Poly(A) Bead 2.0 Selection | X | X |
| PerkinElmer | Normalization & Pooling Protocol | X | X |
| Beckman Coulter® | SPRI® Purification Protocol | X | X |
| Beckman Coulter® | SPRI® Size Selection Protocol | X | X |
| Roche® | SYBR® FAST qPCR Kit | X | |
| Roche® | Library Quant Kit | X | X |
| Roche® | hgDNA Quantification and QC Protocol | X | X |
| Thermo Fisher® | PicoGreen® Quantitation | X | X |
| Thermo Fisher® | Qubit® Quantitation | X | |
| Thermo Fisher® | Quant-iT® Quantitation | X | |
| NuGen® | Encore® Biotin Module | X | |
| NuGen® | Ovation® FFPE WTA System | X | |
| NuGen® | Ovation® Pico WTA System v2 | X |
LIQUID HANDLERS DESIGNED TO MEET YOUR THROUGHPUT REQUIREMENTS
We offer a suite of liquid handlers to offer labs the flexibility of choosing a automation solution based on their throughput needs. Learn more about our liquid handlers optimized for NGS library prep.
For hands-free automated construction
of up to or greater than 96 libraries per day
For the automated construction of up to or
greater than 96 libraries per day
