UDI BARCODES FOR MULTIPLEXING RNA-SEQ LIBRARIES FOR ILLUMINA® SEQUENCING

NEXTFLEX® Unique Dual Index Barcodes

for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0

  • For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
  • Every lot is functionally validated and tested for index purity by sequencing
  • Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For research use only. Not for use in diagnostic procedures.

  • NOVA-512920

       $768

    BARCODES 1-96
    192 rxns

  • NOVA-512921

       $768

    BARCODES 97-192
    192 rxns

  • NOVA-512922

       $768

    BARCODES 193-288
    192 rxns

  • NOVA-512923

       $768

    BARCODES 289-384
    192 rxns

Ready-to-Go UDI Barcodes for RNA-Seq Applications

The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.

For labs who want greater multiplexing capacity, PerkinElmer also offers a set of 1,536 RNA-seq 2.0 UDI barcodes.

Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.

Optimized to Improve Data Integrity

The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.

KIT CONTENTS

  • NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes* (6.25 μM )
  • NEXTFLEX® Primer Mix 2.0** (50 μM )

The 192 rxn kits will be plated column-wise (1-8, 9-16, etc.).

*These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.

** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0; if the primer mix is to be used with any other RNA-seq kits of choice, additional dilutions may be required. For additional guidance, please inquire at [email protected].

SEQUENCES

  • Sequences of NEXTFLEX® RNA-seq 2.0 Unique Dual-Indexed DNA Barcode Indexes – Excel / PDF

MANUAL

Each NEXTFLEX® RNA-seq 2.0 Unique Dual Index barcodes contains 96 unique dual-index barcodes at 6.25 μM, enabling users to multiplex up to 384 RNA-Seq library samples per flow cell lane. Each lot of the NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing. NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes are supplied in 96-well plates, plated column-wise, with each well containing 2 reactions of each barcode. These kits ship on dry ice. The shelf life of each reagent is at least 2 years when stored properly.