ADAPTERS COMPATIBLE WITH ILLUMINA® PLATFORMS FOR DEEP MULTIPLEXING OF UP TO 192 SAMPLES
NEXTFLEX® Dual-Indexed DNA Barcodes
- Enables deep multiplexing of Illumina® DNA libraries for NGS applications
- 8 nt index contained within adapter sequence
- Increase your sequencing scale by pooling up to 192 samples on a single flow cell
- The kits are supplied in a 96-well plate format
- Compatible with Illumina® next-generation sequencing platforms
For research use only. Not for use in diagnostic procedures.
Increase Scale while Reducing Cost
The NEXTFLEX® Dual-Indexed DNA Barcodes are two kits that each contain 96 combinatorial dual indexed barcoded adapters for Illumina® library prep which enable deep multiplexing of NGS sequencing samples compatible with high-throughput automation. They significantly increase scale, while reducing costs by allowing the user to pool up to 192 samples in a single flow cell.
Libraries made using the NEXTFLEX® Dual-Indexed DNA Barcodes can be combined with single index libraries using a dual index sequencing read primer. The NEXTFLEX® Dual-Indexed DNA Barcodes can be used with single, paired-end and multiplex reads, and are compatible with the NEXTFLEX® Rapid DNA-Seq Kit 2.0, the NEXTFLEX® Methyl DNA-Seq Kit, the NEXTFLEX® Cell Free DNA-Seq Kit and other genomic DNA library prep kits.
For larger volume requirements, customized and bulk packaging is available. Please contact [email protected] for further information.
- NNEXTFLEX® Dual-Indexed Adapter (25 µM)
- NEXTFLEX® Primer Mix (12.5 µM)
- NEXTFLEX® Rapid DNA Sequencing Kit 2.0
- NEXTFLEX® Cell Free DNA-Seq Kit
- NEXTFLEX® Methyl Sequencing 1 Kit
What Sequencing Primers Do I Use for Dual-Indexed Libraries?
Libraries made with the NEXTFLEX® Dual-Indexed DNA Barcodes differ from libraries made with the NEXTFLEX® DNA Barcodes in that they have two index sequences, as opposed to a single barcode index. Depending on the sequencing platform and flowcell being used, dual-indexed libraries may require the use of special sequencing primers in place of the primers found in the TruSeq® Cluster Kit.
Sequencing of dual-indexed libraries on the MiSeq® does not require the use of the TruSeq® Dual Index Sequencing Primer Box in addition to the MiSeq® Reagent Kit.
Sequencing dual-indexed libraries on the HiSeq® or GAIIx, however, may require the use of the TruSeq® Dual Index Sequencing Box. The TruSeq® Dual Index Sequencing Box, SR (Illumina® cat. # FC-121-1003) is required when sequencing dual-indexed libraries on a single-read flow cell. In this case, the Index 2 (i5) Sequencing Primer Mix, HP9, must be loaded in position 16 on the flowcell. If sequencing dual-indexed libraries on a paired-end flow cell for HiSeq® or GAIIx, the TruSeq® Dual Index Sequencing Box is not required, as the Index 2 primer is grafted on the flow cell.
In summary, sequencing dual-indexed libraries requires additional primers only if sequencing on a single-read flow cell on the HiSeq® or GAIIx. Sequencing on a paired-end flow cell on the HiSeq® or GAIIx or on the MiSeq® does not require additional primers to be purchased in addition to the typical sequencing primer kit for each sequencer.