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cfDNA and cfRNA Workflow Solutions2019-11-07T18:57:52+00:00

IMPROVING THE EFFICIENCY OF cfDNA AND cfRNA WORKFLOWS

Complete, Automated Solutions

Improving the Efficiency of cfDNA and cfRNA Workflows

Changes in the levels of circulating nucleic acids are associated with certain disease conditions and could be useful for clinical applications, particularly for cancer patients and pregnant women. Significant research effort is being focused on developing clinical research reagents for prenatal disorders and malignant disease monitoring using DNA recovered from cell-free fluids. Cell free DNA (cfDNA) is a sample type of choice due to its quick, very minimally-invasive method of collection that is amenable to analyzing multiple time points and multiple samples at a time when compared to more time-intensive, invasive methods such as tumor biopsy/fine-needle aspiration in cancer or chorionic villus sampling (CVS), or amniocentesis in prenatal testing research. Additional benefits for the use of cfDNA for oncology and other disorders include the provision of a big picture, real time snapshot of a comprehensive disease state and an intra-tumor heterogeneity profile at the time of detection and at relapse or metastasis, as opposed to what a local biopsy may offer.

Circulating DNA can be highly fragmented and present at low concentrations, especially in healthy individuals, which may pose a number of challenges that need to be overcome to achieve reliable for cfDNA extraction. This has led to a need for more efficient methods for extracting, processing, and analyzing cell-free DNA (cfDNA). To meet this need, PerkinElmer has developed a complete, automated workflow for extracting cfDNA and performing subsequent next generation sequencing applications.

cfRNA Biomarkers

Cell-free RNA (cfRNA) also naturally occurs in cell free fluids. Cell-free circulating tumor RNA, or cfRNA shed from tumor cells, may mediate intercellular communication and may be used as biomarkers for the screening or diagnosis of diseases like cancer and the monitoring of therapy.

Improve the Efficiency of Your cfDNA and cfRNA Workflows

Our automated and optimized end-to-end solutions allow labs to minimize errors, reduce hands-on time, and increase throughput and reproducibility. Flexible solutions based on your testing and throughput requirements are available to meet your lab’s needs.

  • cfDNA & cfRNA Literature

Detect miRNA biomarkers in animal biofluids
The NEXTFLEX® Small RNA-Seq kit V3 “consistently performed well with respect to enrichment of miRNA mapping reads in biofluids and tissues” and “exhibited a relatively low quantification bias”.
Coenen-Stass, A.M.L., et al. (2018) Evaluation of methodologies for microRNA biomarker detection by next generation sequencing. RNA Biology. , 15: 8. 15:8, 1133-1145.

SOLUTIONS

cfDNA ISOLATION FROM SERUM & PLASMA

DNA

cfDNA ISOLATION FROM URINE

LabChip chip

VISUAL QC & SIZING OF cfDNA

LIBRARY PREPARATION REAGENTS

Automated Library Prep

AUTOMATED LIBRARY PREPARATION

library quality control

LIBRARY QUALITY CONTROL

For research use only. Not for use in diagnostic procedures.