NextPrep-Mag Urine cfDNA Isolation Kit2018-09-06T02:01:12+00:00

FOR ISOLATION OF CIRCULATING DNA FROM URINE FOR NGS

NextPrep-Mag Urine cfDNA Isolation Kit

  • Ideal for extracting DNA from urine for cell-free DNA analysis
  • Generates highly concentrated libraries using few PCR cycles
  • Automation friendly, mag-bead based format
  • Fast, 30 minute protocol with minimal hands-on time
  • Ideal for NGS library prep
  • No vacuum pumps, vacuum manifolds, or column extenders required

  • NOVA-3826-01

       $159

    (< 1 mL - 4 mL)
    12-50 ISOLATIONS

  • NOVA-3826-02

       $212

    (4 mL – > 20 mL)
    5-25 ISOLATIONS
Download Manual
For research use only. Not for use in diagnostic procedures.
CELL FREE DNA-SEQ

  • NOVA-5150-01

       $312

    for Illumina® Platforms
    8 RXNS
CELL FREE DNA-SEQ

  • NOVA-5150-02

       $1,402

    for Illumina® Platforms
    48 RXNS
CELL FREE DNA-SEQ

  • NOVA-4002-01

       $181

    for Ion Torrent Platforms
    8 RXNS
CELL FREE DNA-SEQ

  • NOVA-4002-02

       $860

    for Ion Torrent Platforms
    48 RXNS

Extracting cfDNA from Urine

The NextPrep-Mag Urine cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from urine, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the NEXTFLEX® Cell Free DNA-Seq Library Prep Kit for Illumina® Sequencing and the NEXTFLEX® Cell Free DNA Library Prep Kit for Ion Torrent Sequencing.

NextPrep-Mag Urine cfDNA Isolation Kit Compared to the QIAamp Circulating Nucleic Acid Kit

The NextPrep-Mag Urine cfDNA Isolation Kit offers a number of advantages over the QIAamp Circulating Nucleic Acid Kit, including speed of cfDNA isolation and ease of automation, while delivering comparable cfDNA yields and higher concentration NGS libraries.

Cell Free DNA Isolation Protocol

The procedure, which can be completed in approximately 30 minutes, includes an initial lysis and DNA binding step, wash steps, and elution of cfDNA from the magnetic beads. A distinguishing feature of the kit is the very rapid magnetic bead attraction steps.

URINE + BINDING SOLUTION + PROTEINASE K + MAGNETIC BEADS (15 MIN)

workflow protocol separator

ATTRACT TO MAGNET & REMOVE FLUID

workflow protocol separator

RAPID WASH STEPS

workflow protocol separator

ELUTE (5 MIN)

workflow protocol separator

TRANSFER cfDNA

KIT CONTENTS

  • cfDNA DNA Proteinase K
  • Urine cfDNA Binding Solution
  • cfDNA Magnetic beads
  • cfDNA Wash Solution 1
  • cfDNA Wash Solution 2
  • cfDNA Elution Solution
  • Control PCR Primers

REQUIRED MATERIALS NOT PROVIDED

  • Ethanol, 100%
  • Vortex mixer
  • Heat block at 55°C
  • Magnetic stands to hold 2 mL microcentrifuge tubes and 15 mL or 50 mL tubes
  • Microcentrifuge or low-speed “picofuge”
  • Nuclease-free 2 mL (1.5 mL and 0.5 mL optional) microcentrifuge tubes and 15 mL tubes
  • Pipettors (P-1000, P-200, P-20) and tips
  • Disposable pipettes (variable sizes depending on sample volume processed)

The NextPrep-Mag Urine cfDNA Isolation Kit For < 1 mL – 4 mL contains sufficient reagents for extraction of DNA from 50 samples of 1 mL urine or less, or 12 samples of 4 mL of urine. The NextPrep-Mag Urine cfDNA Isolation Kit For 4 mL – > 20 mL contains sufficient reagents for extraction of DNA from 25 samples of 4 mL urine or less, or 5 samples of 20 mL of urine. The kit components are stable for at least 12 months from time of receipt, when properly stored.

  • NextPrep-Mag cfDNA Isolation Kit (for use with plasma)
  • NEXTFLEX® Cell Free DNA-Seq Kit for Illumina® Sequencing
  • NEXTFLEX® Cell Free DNA-Seq Kit for Ion Torrent Platforms
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