In this study, an established biospecimen provider to processed a biopsy from a renal cell carcinoma (RCC) tumor using their established SOPs to dissociate fresh tumor tissue, perform flow cytometry, and cryopreserve the sample or capture single cells with the HIVE™ scRNAseq Solution for single cell RNA sequencing. The HIVE™ scRNAseq protocol was easily adopted into their standardized operations.
Samples stored, shipped and processed using the HIVE™ scRNAseq Solution precisely matched flow cytometry from freshly dissociated tissue without any sample bias. The HIVE™ stabilized all cells in the sample and yielded high integrity scRNAseq data through 20 weeks’ storage. Cryopreservation immediately damaged the sample and destroyed precious immune cells, including T cells, monocytes, and macrophages through 20 weeks’ storage, biasing scRNAseq data obtained from solid tumor tissues.