See What Others are Saying About the NEXTFLEX® Chemistry
Six independent, peer-reviewed studies were published in 2018 and 2019 comparing small RNA sequencing data obtained using commercially available small RNA-seq library prep kits in human samples. The NEXTFLEX® Small RNA-Seq kit was recommended in every study because of the low bias and consistent results obtained using the kit.
Coenen-Stass, A.M.L., et al. (2018) Evaluation of methodologies for microRNA biomarker detection by next generation sequencing. RNA Biology. 15: 8. 15:8, 1133-1145. doi: 10.1080/15476286.2018.1514236.
Dard-Dascot, C., et al. (2018) Systematic comparison of small RNA library preparation protocols for next-generation sequencing. BMC Genomics 19(118), doi:10.1186/s12864-018-4491-6.
Giraldez, M. D., Spengler, R. M., Etheridge, A., Godoy, P. M., Barczak, A. J., Srinivasan, S., . . . Tewari, M. (2018). Comprehensive multi-center assessment of small RNA-seq methods for quantitative miRNA profiling. Nature Biotechnology. doi:10.1038/nbt.4183.
Ku, A., Ravi, N., Yang, M., Evander, M., Laurell, T., Lilja, H., & Ceder, Y. (2019). A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated extracellular vesicle enrichment by acoustic trapping to microRNA sequencing. Plos One, 14(10). doi: 10.1371/ journal.pone.0224604.
Wright, C., Rajpurohit, A., Burke, E. E., Williams, C., Collado-Torres, L., Kimos, M., . . . Shin, J. H. (2018). Comprehensive assessment of multiple biases in small RNA sequencing reveals significant differences in the performance of widely used methods. bioRxiv 445437. doi:10.1101/445437.
Yeri, A., et al. (2018) Evaluation of commercially available small RNASeq library preparation kits using low input RNA. BMC Genomics 201819:331. doi: 10.1186/s12864-018-4726-6.