Materials and Methods
The NEXTFLEX® NGS Hybridization Panels contain library prep reagents, index barcodes, blockers, baits, streptavidin-beads, hybridization and wash buffers, and post-capture amplification reagents. The kit is designed for approximately 2.5-hour precapture DNA library construction, with enzymatic fragmentation included, from as little as 1 ng DNA, and is optimized for subsequent target enrichment prior to sequencing. The kit can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The recommended minimum input for hybrid capture is 100 ng of starting material into the pre-capture library prep.