INTEGRATED SAMPLE STORAGE & SINGLE-CELL PROFILING SOLUTION

HIVE scRNAseq Solution

Expand the Possibilities for Single Cell Transcriptional Profiling

Biological resolution at the level of individual cells is powering the next phase of precision health. Developed by Honeycomb Biotechnologies, the HIVE™ scRNAseq Solution integrates sample storage and single cell profiling into a complete workflow, solving the issues that limit single cell RNA analysis by:

  • Enabling multi-site and multi-timepoint sample collection

  • Maintaining sample integrity through storage, shipping, and processing

  • Increasing the recovery rates of fragile cells

  • Facilitating loading of larger sample volumes

  • Delivering flexible and scalable workflows by enabling batch processing

  • Removing the need for specialized instrumentation

The HIVE™ Collector is a portable, handheld, single-use device that enables gentle capture, robust storage, and easy processing for the analysis of single-cell samples. The HIVE™ scRNAseq Solution will expand single-cell analysis in basic, translational, pre-clinical, and clinical research applications.

Visit Honeycomb’s Website to access HIVE scRNAseq™ protocols and technical information.

Request a quote to expand your possibilities with enhanced single cell transcriptional profiling
For research use only. Not for use in diagnostic procedures.
HIVE single cell RNA-seq Collector

THE HIVE™ scRNAseq SOLUTION WORKFLOW

single cell rna-seq workflow

Enabling Multi-site & Multi-timepoint Sample Collection

The HIVE™ scRNAseq solution is a combined sample storage and single-cell profiling system. The HIVE™ Collector delivers versatility by enabling sample collection at different sites or at different timepoints and sample processing at the same location where they are collected or at a centralized processing laboratory.

Easy to Use

The HIVE™ scRNAseq solution incorporates reagents and a single-use, handheld, shippable HIVE™ Collector that can be used in almost any lab environment. No specialized equipment is required to process the samples from single cells through library preparation and the workflow can be run in most labs.

SCENARIO 1:
A clinical researcher receives bone marrow biopsies at odd hours, making it challenging to process them fresh and at the same time for ideal data quality. Additionally, the specialized equipment required for her current single cell solution is not always available. With the HIVE™ Collector, she can capture her samples at any time, then store her cell-loaded HIVE™ device(s) until all samples are ready for processing together, as if they had been processed fresh.

With a sample storage solution built into the capture and processing workflows, the HIVE™ Collectors provide a stable environment to arrest cells in their current states and protect the sample over time.

Maintaining Sample Integrity

Cell quality is not compromised when using the HIVE™ Collector during storage, shipping or processing. As can be seen in Figure 1, single cell libraries prepared from fresh cells and cells stored at -20°C for 14 days prior to processing have similar profiles, showing no loss of cell population diversity, number of transcripts or genes detected.

Figure 1. Comparison between samples processed while fresh or stored at -20°C for 14 days prior to processing.
Maintaining single cell RNA-seq Sample Integrity

SCENARIO 2:
A researcher has been attempting to recover neutrophils from whole blood samples. He has been struggling to recover neutrophils with current microfluidic platforms in the lab, but the HIVE™ Collector’s gentle capture of cells enables him to generate data for neutrophils and other fragile cell types.

The HIVE™ scRNAseq solution delivers comprehensive biological representation,
including fragile and sparse cells.

Increasing the Recovery Rates of Fragile Cells during single cell RNA-seq

Increasing the Recovery Rates of Fragile Cells

Cells isolated using the HIVE™ Collector settle gently into microwells within a matter of minutes. This change enables the analysis of cells that are typically difficult to analyze either because of their fragile membrane structures or because they have been stimulated or transfected which can induce fragility. Figure 2 shows the HIVE™ scRNAseq workflow is able to recover granulocytes which are difficult to detect with other platforms because of their fragility.

Figure 2. Illustrates 24,720 cells were captured in six HIVE™ Collectors. The cell types processed include granulocytes such as neutrophils, eosinophils, and basophils.

SCENARIO 3:
A researcher has 10 mL of fresh urine with only 10,000 cells total. With their current single-cell RNA-seq technology, they have to concentrate 10 mL to less than 100 µL. With the HIVE™ Collector, they can load their entire sample without any risk of losing cells due to concentration before capture.

Facilitating Loading of Larger Sample Volumes

Other systems for single cell analysis only allow small sample loading volumes, typically around 50 μL. To ensure you are not missing critical information available in the primary sample, the HIVE™ scRNAseq solution allows you to load much larger volumes of up to 4 mL.

Delivering Flexible & Scalable Workflows

The HIVE™ scRNAseq workflow reduces experimental and inter-site variability seen in other single cell RNA-seq analysis platforms. The workflow also delivers flexibility of processing location and throughput. The samples can be easily processed where they are collected or stored and shipped to a central facility for processing. By allowing safe sample storage, samples can be batched to improve lab efficiency.

CONTENTS OF KITS

If you need the entire HIVE™ scRNAseq Solution you should buy:            
HIVE™ scRNAseq Complete Kit

  • The HIVE™ scRNAseq Complete Kit is the combined HIVE™ Sample Capture and HIVE™ Processing Kits for the capture, processing, and library prep of single-cell samples using the HIVE™ device and corresponding reagents for 8 samples.

HIVE™ scRNAseq Starter Bundle

  • Index plate (96 rxns)
  • HIVE™ accessories (1 x closure tool, 1 x filter plate adapter)
  • Cell surrogates (2 rxns, needed for training only)
  • Molecular controls (2 rxns, needed for training/verification only)
  • Vacuum kit (1 x container vac trap, 1 x assembled gauge, 1 x manifold)
  • Plate kit (1 x 96-well filter plate, 1 x 96 deep well plate, 5 x 96 full height PCR plate, foil, and clear plate seals)
  • Lysis boxes (2 x reusable boxes)

If you need the capture portion of the HIVE™ scRNAseq Solution you should buy:            
HIVE™ scRNAseq Sample Capture Kit

  • HIVE™ Collectors & Reagents for 8 samples

If you need the processing and library prep portion of the HIVE™ scRNAseq Solution you should buy:   

HIVE™ scRNAseq Processing Kit

  • Parts & Reagents to process cell-loaded HIVE™ Collectors and construct libraries for 8 samples.

HIVE™ scRNAseq Index Plate

  • Index primers to multiplex 96 libraries

HIVE™ scRNAseq Starter Bundle

  • Index plate (96 rxns)
  • HIVE™ accessories (1 x closure tool, 1 x filter plate adapter)
  • Cell surrogates (2 rxns, needed for training only)
  • Molecular controls (2 rxns, needed for training/verification only)
  • Vacuum kit (1 x container vac trap, 1 x assembled gauge, 1 x manifold)
  • Plate kit (1 x 96-well filter plate, 1 x 96 deep well plate, 5 x 96 full height PCR plate, foil, and clear plate seals)
  • Lysis boxes (2 x reusable boxes)

HIVE™ Sample Analysis
BeeNet™ Software

Single Cell Collection FAQs:

Q. How long can I store a cell-loaded HIVE™ Collector ?
A. The HIVE™ Collector is currently verified for up to two weeks of storage at -20°C, and has been successfully tested with filtered blood for up to five months of storage at -20°C.

Q. How many samples can I process at once and how long does it take?
A. The HIVE™ workflow offers a high degree of flexibility, as each sample is independent. 1 to 24 samples can be worked with in parallel and can be processed and ready for sequencing in 1.5 days.

Q. How many cells can be loaded per HIVE™ Collector?
A. Loading ~15,000 cells per HIVE™ Collector is recommended, though the loading range is 500-30,000 cells per device.

Q. What is the sample loading volume?
A. The HIVE™ Collector’s loading capacity is 1 – 4 mL. Loading 1 mL is recommended. If your sample volume is below 1 mL, you can use enough fresh cell media to adjust the sample volume before loading.

Q. What sample types can I use?
A. The HIVE™ scRNAseq solution can be used with eukaryotic cells containing mRNA transcripts with 3’ poly-A tails. A variety of mammalian cell types have been tested; verified primary cells and cell lines include human filtered blood, PBMCs, and mouse splenocytes.

Sequencing & Software FAQs:

Q. What sequencing platform do you recommend?
A. The HIVE™ scRNAseq Solution is compatible with Illumina® sequencing platforms capable of custom dual index paired-end sequencing. The Illumina® NovaSeq® and NextSeq® 500/550 platforms are reccomended.

Q. Are custom sequencing and index primers provided with the kit?
A. Yes, they are included and are compatible with Illumina® NovaSeq® platform v1.5 and the Illumina® NextSeq® platform v2.0 (or later).

Q. What analysis software is provided?
A. Honeycomb’s proprietary BeeNet™ software is available to customers free of charge. Please visit the Honeycomb® Customer Portal for more information.

Request more information about the HIVE™ scRNAseq Solution

For research use only. Not for use in diagnostic procedures.