CONTENTS OF KITS
HIVE™ Sample Capture
HIVE™ scRNAseq Sample Capture Kit
HIVE™ Sample Processing
HIVE™ scRNAseq Processing Kit
- Reagents to process HIVE™ devices and construct libraries for 8 samples.
HIVE™ scRNAseq Index Plate
- Index primers to multiplex 96 libraries
HIVE™ scRNAseq Starter Bundle
- Index plate (96 rxns)
- HIVE™ accessories (1 x closure tool, 1 x filter plate adapter)
- Cell surrogates (4 rxns, needed for training only)
- Molecular controls (4 rxns, needed for training/verification only)
- Vacuum kit (1 x container vac trap, 1 x assembled gauge, 1 x manifold)
- Plate kit (1 x 96-well filter plate, 1 x 96 deep well plate, 5 x 96 full height PCR plate, foil, and clear plate seals)
- Lysis boxes (2 x reusable boxes)
HIVE™ Sample Analysis
Single Cell Collection FAQs:
Q. How long can I store a cell-loaded HIVE™ device?
A. The HIVE™ device is currently verified for up to two weeks of storage at -20°C, and has been successfully tested with filtered blood for up to five months of storage at -20°C.
Q. How many samples can I process at once and how long does it take?
A. The HIVE™ workflow offers a high degree of flexibility, as each sample is independent. 1 to 24 samples can be worked with in parallel and can be processed and ready for sequencing in 1.5 days.
Q. How many cells can be loaded per HIVE™ device?
A. Loading ~15,000 cells per HIVE™ device is recommended, though the loading range is 500-30,000 cells per device.
Q. What is the sample loading volume?
A. The HIVE™ device’s loading capacity is 1 – 4 mL. Loading 1 mL is recommended. If your sample volume is below 1 mL, you can use enough fresh cell media to adjust the sample volume before loading.
Q. What sample types can I use?
A. The HIVE™ scRNAseq solution can be used with eukaryotic cells containing mRNA transcripts with 3’ poly-A tails. A variety of mammalian cell types have been tested; verified primary cells and cell lines include human filtered blood, PBMCs, and mouse splenocytes.
Sequencing & Software FAQs:
Q. What sequencing platform do you recommend?
A. The HIVE™ scRNAseq Solution is compatible with Illumina® sequencing platforms capable of custom dual index paired-end sequencing. The Illumina® NovaSeq® and NextSeq® 500/550 platforms are reccomended.
Q. Are custom sequencing and index primers provided with the kit?
A. Yes, they are included and are compatible with Illumina® NovaSeq® platform v1.5 and the Illumina® NextSeq® platform v2.0 (or later).
Q. What analysis software is provided?
A. Honeycomb’s proprietary BeeNet™ software is available to customers free of charge. Please visit the Honeycomb® Customer Portal for more information.