SAFELY COLLECT AND STORE HEPATOCYTES

HIVE™ Single-cell RNAseq Hepatocyte Profiling without Fearing Total Sample Loss

Liver samples^1,2 are notoriously difficult for single-cell platforms. About 80% of liver cells are hepatocytes,³ cells that are large enough to clog microfluidic devices while fragile enough to be destroyed in shear flow. Given the high risk of total failure, researchers often freeze such tissues and isolate nuclei for RNAseq, compromising the amount of mRNA recovered and leading to sparse data.

The HIVE™ technology offers new capabilities to perform scRNAseq from freshly digested liver samples. With gravity or a low-speed spin, single hepatocytes can gently settle into HIVE™ picowells (60 µm size). Extremely large cells, clumps, or debris that can clog other technologies are washed away from the HIVE™ device, and isolated hepatocytes are ready for whole-cell scRNAseq or can be stably frozen for months on end.

Study Parameters

Seamless HIVE™ Workflow

• Freshly dissociated cells from mouse livers
  • 2 healthy samples, 1 tumor biopsy
  • Enriched for hepatocytes (20–50 mm)
• Captured single cells with the HIVE™ scRNAseq Solution
  • Stored at -20°C for 1.5 weeks before generating scRNAseq libraries

scRNAseq metrics*

*median values