IMPROVING THE EFFICIENCY OF cfDNA & cfRNA WORKFLOWS

Complete, Automated Solutions

Improving the Efficiency of cfDNA and cfRNA Workflows

Research is being focused on developing clinical research reagents in the fields of prenatal disorders and malignant disease monitoring using DNA recovered from cell-free fluids. Cell-free DNA (cfDNA) is a sample type of choice due to its quick, minimally invasive method of collection that is amenable to analyzing multiple time points and multiple samples at a time.

Circulating DNA can be highly fragmented and present at low concentrations, which may pose a number of challenges that need to be overcome to achieve reliable cfDNA extraction. This has led to a need for more efficient methods for extracting, processing, and analyzing cfDNA. To meet this need, PerkinElmer has developed a complete, automated workflow for extracting cfDNA and performing subsequent next generation sequencing applications.

cfRNA Biomarkers

Cell-free circulating tumor RNA, or cfRNA shed from tumor cells, may mediate intercellular communication and may be used as biomarkers for diseases and monitoring of therapies.

Improve the Efficiency of Your cfDNA and cfRNA Workflows

Our automated and optimized end-to-end solutions allow labs to minimize errors, reduce hands-on time, and increase throughput and reproducibility. Flexible solutions based on your testing and throughput requirements are available to meet your lab’s needs.

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High-Throughput, End-to-End Cell Free DNA Analysis Workflow from Plasma

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Automated Circulating Cell-Free DNA Purification with the chemagic™ 360 Instrument

The NEXTFLEX^® Small RNA-Seq kit V3 “consistently performed well with respect to enrichment of miRNA mapping reads in biofluids and tissues” and “exhibited a relatively low quantification bias”.

Coenen-Stass, A.M.L., et al. (2018) Evaluation of methodologies for microRNA biomarker detection by next generation sequencing. RNA Biology. , 15: 8. 15:8, 1133-1145.