TARGET SEQUENCE ENRICHMENT Combined PGT with Target Sequence Enrichment Preimplantation genetic testing for monogenic diseases (PGT-M) has been applied for many years and it can be performed for any genetic disease that can be diagnosed. With the development of Whole Genome Amplification (WGA) technologies, it has become possible [...]
NON-INVASIVE PGT-A WEBINAR PerkinElmer PG-Seq™ Rapid Non-Invasive PGT-A Webinar Non-invasive PGT-A might be the next wave in preimplantation genetic testing, offering similar results to biopsy-based PGT-A without the need to invasively biopsy the embryo to achieve results. PGT-A has been shown to improve pregnancy and live-birth rates, decrease [...]
NON-INVASIVE PGT-A Non-invasive PGT-A: Where we are and where we are going Preimplantation genetic testing for aneuploidy (PGT-A) aims to rank embryos for clinical transfer by their ploidy, and relies on evaluating the chromosomal content of a representative for the entire conceptus. Historically, that proxy has been a [...]
Do We Have Enough Data (yet) on PGT-A? | I’ve been involved in the Preimplantation Genetic Testing field since my first case in 1992. I’ve seen it grow and change over time in response to the latest publications and I’ve seen the tests evolve from polymerase chain reaction (PCR) through fluorescence in situ hybridization (FISH), microarrays, and next generation sequencing (NGS).
High-throughput, reliable technologies for PGT-A | The field of genetics continues to grow at an unforeseen pace. Sequencing instrument manufacturers are rising to the challenge by offering larger and larger sequencing platforms capable of sequencing the entire human genome in just a few days with the promise of achieving the $1,000 genome. The growth of this market doesn’t look like slowing down any time soon.
How to Design PCR Primers for Multiplex Assays | Good primer design is essential for successful, specific PCR amplification with high yield. For PCR primers to efficiently amplify DNA template in a PCR they should be carefully designed so the optimal conditions for them to amplify their target are as equivalently matched as possible.
Here I am, a woman undergoing IVF to try and have the child that my partner and I so desperately want to have, and I find out that the majority of my embryos from each IVF cycle do not have the correct number of chromosomes!
Preimplantation Genetic Testing Podcast | Listen to the It’s In Our Blood podcast recorded by Pathology Technology Australia. In this podcast Dr. Harton describes the basic concept of preimplantation genetic testing and the current and likely future utilization of the technology.
Comparing the Reproducibility of Different Aneuploid Findings | This webinar describes a study that used two independent next-generation sequencing (NGS) platforms to gain insight into the impact of different types of aneuploidies during preimplantation genetic testing....