Revvity Applied Genomics

Tips on Establishing a Reliable Cell-Free DNA Workflow from Plasma Samples

Analyzing circulating cell free DNA (ccfDNA) offers a non-invasive, dynamic window into the body’s physiological state and its use as a biomarker is gaining rapidly. However, multiple challenges remain with establishing its routine application. This is mainly due to its low levels, high rates of fragmentation, and preanalytical variabilities associated with sample collection, cfDNA extraction and quantification. Here, we go through some tips on how one can establish a reliable cfDNA workflow from plasma samples.

WEBINAR: Key Considerations in Automated Nucleic Acid Extraction for Pathogen and Genetic Screening Workflows

Nucleic acid extraction lies at the beginning of almost every molecular biology-based workflow. Although the principle behind it is simple, when done poorly, it can have a significant impact on final experimental results. If working with patient samples, maintaining sample integrity becomes even more essential and reliable results are paramount to ensuring a fast and accurate diagnosis.

Automating Twist Target Capture

Scale NGS Projects with the Sciclone® G3 NGSx Automated Liquid Handler | Listen to Dr. Renata Pellegrino, the Laboratory Director in the Center for Applied Genomics at the Children’s Hospital of Philadelphia, discuss how her team has combined Sciclone® technology and Twist® Bioscience’s target capture chemistry to optimize her next-generation sequencing (NGS) workflow.

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