THE GENOMICS ENTHUSIAST
Enabling HIV Epidemiology
ENABLING HIV EPIDEMIOLOGY Simplifying HIV Variant Tracking with Low Throughput Automation "Lab A" is an epidemiology lab tracking variants of the human immunodeficiency virus (HIV). They collect samples from approximately 16 people a day, amplify the whole viral genome with specific primers and the amplicons obtained are sequenced. An amplicon-based approach for whole genome sequencing (WGS) facilitates the analysis of [...]
Reducing Errors From Low-throughput Library Prep
10 THINGS THAT CAN (AND DO) GO WRONG WITH MANUAL LIBRARY PREP How To Minimize Human Error in Low Throughput NGS Workflows There are many steps during library preparation where human error can be introduced. These errors can negatively impact the reliability and accuracy of sequencing data and waste valuable samples. In this post we describe a few [...]
Tips on Establishing a Reliable Cell-Free DNA Workflow from Plasma Samples
Analyzing circulating cell free DNA (ccfDNA) offers a non-invasive, dynamic window into the body’s physiological state and its use as a biomarker is gaining rapidly. However, multiple challenges remain with establishing its routine application. This is mainly due to its low levels, high rates of fragmentation, and preanalytical variabilities associated with sample collection, cfDNA extraction and quantification. Here, we go through some tips on how one can establish a reliable cfDNA workflow from plasma samples.
Pandemic Preparedness and Prevention
SURVEILLANCE PLANS ARE ESSENTIAL FOR PANDEMIC PREPAREDNESS AND PREVENTION The main lesson being learned from the COVID-19 pandemic is the importance of detecting cases early, following up on close contacts and preventing further disease spread for as long as possible. Other new and emerging diseases can affect us anywhere and at any time. The European Centre for Disease Prevention and [...]
Meeting IVDR Requirements and More with New CE-IVD chemagic™ Nucleic Acid Purification Kits
Meeting IVDR Requirements and More with New CE-IVD chemagic™ Nucleic Acid Purification Kits | In vitro diagnostic (IVD) manufacturers have been busy bees in the past months, working intensively to meet the new European Union regulations known as the European In Vitro Diagnostic Medical Device Regulation (EU IVDR) that come into play 26th May 2022.
Automated Normalization of NGS Libraries
Incorporating Normalization Beads into your NGS Workflow | Normalization is the process by which NGS libraries of different concentrations are diluted to the same final concentration before pooling and sequencing.
How to Reduce Static Electricity on Your Liquid Handlers
Reduce Static Electricity on Liquid Handlers | Throughout dry winter periods the air humidity changes. This change can cause a buildup of static electricity in the air and on plastic liquid handling tips for automated liquid handlers.
Preventing Future Pandemics
Timely Identification, Tracking and Mitigation of Future Outbreaks | We could have prevented the SARS-CoV-2 outbreak from developing into a pandemic, but we did not have an adequate surveillance system that could detect novel threats, particularly those that can be carried by asymptomatic carriers.
Non-Invasive Preimplantation Genetic Testing for Embryology
Bill Venier, M.Sc, TS, ELD (ABB) and Deb Venier, MS, TS, of World Embryology Skills and Training (W.E.S.T.) draw from their many years of experience in the embryology lab to discuss their perspective on non-invasive PGT-A.
How to Load Barcode Indexes in Illumina® Experiment Manager
How to Load Barcode Indexes in Illumina® Experiment Manager | The Illumina® Experiment Manager (IEM) is an app used to create valid sample sheets for some Illumina® sequencers. Although sample sheets can be generated manually, this tool minimize the risk of errors.
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