Archives for April 2019

Managing the Risks of CRISPR

Managing the Risks of CRISPR | The use of CRISPR/Cas9 to edit the eukaryotic genome is having a dramatic impact on virtually every field of biotechnology, from crop science to tumor biology (1). The two key features that set this genome modification system apart from other genomic tools are its efficiency and specificity. However, recent articles...

When to Size Select

Fragmentation Method Effects on Size Selection Needs | Fragmentation of nucleic acids prior to library construction is required by the majority of next-generation sequencing platforms. Available methods for fragmenting high quality genomic DNA vary in their ability to focus sheared nucleic acids to a tight average fragment size. Broadly distributed shearing profiles are obtained when using probe-based...